Alkanal monooxygenase

Аlkanal
monooxygenase
EC 1.14.14.3
Made by
Katherine Bukina,
4 year student
Krasnoyarsk, 2011
INTRODUCTION
EC 1.14.14.3
Organism Vibrio harveyi
1. Oxidoreductases
1.14 Acting on paired donors, with O2 as oxidant and
incorporation or reduction of oxygen. The oxygen
incorporated need not be derived from O2
1.14.14 With reduced flavin or flavoprotein as one
donor, and incorporation of one atom of oxygen into
the other donor
1.14.14.3 Alkanal monooxygenase (FMN)
Enzyme review EC 1.14.14.3
NAMES
Accepted
name:
alkanal monooxygenase (FMN-linked)
Vibrio fischeri luciferase
aldehyde monooxygenase
Other names:
bacterial luciferase
luciferase
Systematic
name:
Enzyme review EC 1.14.14.3
alkanal,reduced-FMN:oxygen
oxidoreductase (1-hydroxylating,
luminescing)
REACTION
Enzyme review EC 1.14.14.3
REACTION
STRUCTURE
Люцифераза представляют собой αβ-гетеродимер,
состоящий из двух гомологичных субъединиц (α и β),
молекулярная масса которых соответственно 40 и 35 кДа.
Изгибы α- и β-субъединиц
очень похожи, оба
принимают форму
скрученного бочонка ([β/α]8).
Две субъединицы связаны
большой соприкасающейся
поверхностью. Активный
центр фермента расположен,
главным образом, на αсубъединице.
STRUCTURE
Subunit A
Subunit A
Subunit B
Subunit B
LIGANDS AND METALS
STATISTICS
STATISTICS
STATISTICS
STATISTICS
STATISTICS
STATISTICS
INTERESTING ARTICLES
• Tu SC. Activity coupling and complex formation between
bacterial luciferase and flavin reductases. Photochem Photobiol
Sci. 2008 Feb;7(2):183-8.
• Mark S. Waidmann Bacterial luciferase reporters The Swiss
army knife of molecular biology Bioengineered Bugs 2:1, 8-16;
January/February 2011; © 2011 Landes Bioscience
• Yamasaki S, Yamada S, Takehara K. Inhibition of
electrochemically controlled bioluminescence of bacterial
luciferase by n-alkyl alcohols. Anal Sci. 2011;27(4):357.
• Close DM, Hahn RE, Patterson SS, Baek SJ, Ripp SA, Sayler GS.
Comparison of human optimized bacterial luciferase, firefly
luciferase, and green fluorescent protein for continuous imaging
of cell culture and animal models. J Biomed Opt. 2011
Apr;16(4):047003.
SOURCES
1. http://www.rcsb.org
2. http://www.ncbi.nlm.nih.gov/pubmed
3. http://www.ebi.ac.uk
4. http://www.gopubmed.org
A time to ask something!
Questions?
Thank you for
your attention!

Adieu