Влияние адаптации к коротким стрессорным воздействиям и

, 2009,
8,
2
N-L-
.
«
»
.
N-
-L-
.
(GSH)
(GSSG)
.
,
(
(
)
)
.
13%
GSH/GSSG
19%,
46%.
,
,
GSH
N-
GSSH.
-L-
,
,
,
,
.
:
, N.
1
-L-
,
,
, 2009,
8,
2
Abstract. The work was aimed at the investigation of adaptation influence to
short stress and preliminary N-acethyl-L-cystein injection on the poststressory
changes of lipid peroxidation activity and redox –system of glutathione. The
cellular redox state has been estimated according to reduced (GSH) and oxidized
(GSSG) glutathione ratio.The concentration of nitrates and nitrites, activity of
lipid peroxidation (LPO) and general antioxidant activity (AOA) of bloods
plasma have been defined in this work. The LPO activation (13%), AOA
inhibition of blood plasma (19%) and oxidized glutathione accumulation and the
reduction of GSH/GSSG ratio (46%) has been observed in the group of stressed
animals.
Adaptation to the short stressor influences has completely prevented the
poststressory activation of lipid peroxidation, the reduction of antioxidant
activity, accumulation of nitric monoxide degradation products and restored the
GSH/GSSG ratio. Preliminary N-acethyl-L-cystein injection possessed by the
similar properties as adaptation to the short stressory influences, i.e. prevented the
poststressory changes in cellular redox-state, reduction of antioxidant activity,
activation of lipid peroxidation and superfluous nitric monoxide accumulation.
:
, 210023, .
,
. 37-07-54 -
,
.
, 27,
,
.
,
.
,
.
(
(GSSG)
,
(GSH)
+
,
,
2
+
) [9, 10].
, 2009,
8,
2
(GSSG/GSH)
,
«
»
.
,
GSSG/ GSH
,
,
,
,
. [9,10].
,
.
,
«
»
N-
-L-
.
,
N-
-L-
N-
-L-
,
,
)
,
[5].
–
.
148
6–
.
8
:
3
, 2009,
8,
2
22°
: 1-
–5
., 2-
– 10
2-
., 3-
– 15
.
.
.
N-
40
1
-L[4].
.
,
[2].
-06
(
).
,
–
.
.
(Imax,
,
),
(S,
)
,
tg2 –
,
[6].
,
[11].
1,10-
.
,
,
(0; 62,5; 125,0;
4
, 2009,
250,0
8,
g/ml)
2
(0; 25,0; 50,0; 100,0
g/ml)
.
2,4N-(2,4-
,
)
S.
20
Zorbax NH2 250 4,6
,
5
Agilent 1100 (
G1311A,
G1315B,
G1316A,
(
) G1313A).
.
(
,
).
,
Agilent
ChemStation
for
LC
3D.
Microsoft Excel 2000, STATISTICA 6.0.
t.
.
p<0,05.
(I max)
13%,
19% (S,
)
10% (
5
tg 2 ,
.1).
, 2009,
8,
2
1
(n=9)
6
(n=7)
(n=10)
I max,
1,137±0,042
S,
10,4±0,3
tg 2α
-0,241±0,006
1,281±0,040*
1,163±0,042
1,123±0,091
12,4±0,3*
11,0±0,2
10,7±0,7
-0,268±0,007*
-0,258±0,030
-0,259±0,049
(n=9)
:* – p<0,05
; n-
.
,
.
NO2/NO3
53% (
,
.2).
,
.
(
.2).
2
(n=10)
6
(n=11)
(n=7)
(n=7)
: * – p<0,05
NO2/NO3,
27,5±1,2
42,0±1,2*
34,2±2,7*
28,9±1,3
.
; n-
,
,
,
.
6GSH
6
GSSG
, 2009,
8,
2
,
46%
(
.3).
3
,
,
GSH
(n=11)
(n=10)
(n=8)
(n=7)
: *– <0,029
GSSG
521±53
424±76
438±40
509±79
166±25
176±21
126±14
210±58
; n-
,
GSH/
GSSG
4,29±0,78
2,29±0,34*
3,53±0,31
3,67±1,5
.
.
,
,
,
1
N-
-L-
,
,
(
. 4, 5).
4
N-
-L-
(n=9)
6
(n=7)
N-
-L-
(n=7)
N-
-L+
: * – p<0,05
(n=7)
I max,
1,137±0,042
1,281±0,040*
S,
10,4±0,3
12,4±0,3*
1,179±0,040
11,0±0,2
1,072±0,050
; n-
10,3±0,5
7
tg 2α
-0,241±0,006
0,268±0,007*
0,279±0,030*
-0,244±0.030
.
, 2009,
8,
2
5
N-
NN-
-L-
(n=10)
6
(n=11)
-L(n=7)
-L+
: * – p<0,05
NO2/NO3,
27,5±1,2
42,0±1,2*
26,6±2,6
28,9±2,5
.
(n=7)
; n-
N-
-L-
,
N-
-L-
,
,
.
N(
-L-
,
. 6).
6
,
,
N-
GSSG
GSH
NN-
(n=11)
(n=10)
-L(n=7)
-L+
: *– <0,029
-L-
521±53
424±76
483±92
508±63
; n-
(n=7)
GSH/ GSSG
166±25
176±21
154±24
190±42
4,29±0,78
2,29±0,34*
3,37±0,62
4,04±1,67
.
,
N-
-L-
,
,
.
8
,
, 2009,
8,
2
,
GSH
GSSG
GSSG.
,
GSSG/GSH
,
(AP-1, NF-kB, HIF-1) [9, 10].
,
,
.
,
,
.
,
[8].
,
,
,
NO [7].
,
,
,
.
NO
,
S-
,
NO [1]
.
NO
,
(
HSP70 [3],
[3],
[3],
.
9
, 2009,
8,
2
GSH
N-
GSH/GSSG.
-L,
,
.
N-
-L(
,
–
),
,
.
,
,
N-
-
L,
)
,
,
,
,
.
1.
,
,
GSH
2.
N-
GSSH.
-L-
,
,
,
,
.
10
, 2009,
1.
, .
2
.
/ .
2.
8,
S-
.
,
//
–
. – 1998. – . 63. – . 924-938.
.
NO3
NO2
/
. .
//
.–
. .
,
, 1999. – C. 274-277.
3.
?
/
. .
[
.]
//
. – 1998. – . 126. – . 274-277.
4.
N-
/ . .
[
.] //
:
:
.
.-
5.
.
.–
, . .
.
6.
, 2002. – . 71-76.
N-
/
II
-L-
. .
,
. – 2005. –
-
. .
,
. .
//
4. – . 48-50.
, . .
/
. .
,
. .
.–
.:
. –
:
». – 1975. – 127 .
7.
,
/
8.
.
. .
.
,
. .
, 1988. – 252 .
, .
/ . .
.
.– 1994. – . 80,
9.
,
//
.
.
4. – . 65-72.
, . .
:
. .
/
, 1999. – 149 .
11
. .
. –
, 2009,
8,
2
10. Schafer, F. Q. Redox environment of the cell as viewed through the redox
state of the glutathione disulfide / glutathione couple / F. Q. Schafer, G. R. Buettner
// Free Radic. Biol. Med. – 2001. – Vol. 30, N 11. – P. 1191-1212.
11. Yoshida, T. Determination of reduced and oxidized glutathione in
erythrocytes by high-performance liquid chromatography with ultraviolet
absorbance detection / T. Yoshida // Journal of Chromatography B. – 1996. –
678. – . 157-164.
12